![]() ![]() Basic considerationsĪ comprehensive analysis of glucose metabolism in mice from four commonly used inbred strains (C57BL/6, 129X1/Sv, FVB/N and DBA/2) demonstrated strain-dependent differences in insulin action, the neuroendocrine response to hypoglycaemia, and insulin secretion ( Berglund et al., 2008). ![]() These include basic considerations when designing and interpreting experiments, as well as consensus recommendations on choosing the appropriate test, performing the test(s), and describing methods and results. ![]() In this article, we discuss issues pertaining to the standardization of methods for assessing glucose metabolism in mice. This will provide authors and reviewers the necessary information for interpreting results that might differ as a result of differences in methodology. Guidelines must also be established for the description of methods and presentation of results from metabolic tests. However, certain parameters can be standardized, regardless of where tests are conducted. It is recognized that it will not be possible for all metabolic tests to be performed in the exact same manner across different laboratories, as the degree of technical expertise and the availability of equipment and reagents will never be consistent from one laboratory to another. To this end, guidelines must be established for performing tests of glucose metabolism. An important goal of the Mouse Metabolic Phenotyping Center (MMPC) Consortium ( ) is to propose standard methodologies for these key metabolic techniques. The miniaturization of metabolic techniques for use in the mouse has resulted in important advances in our understanding of the pathophysiology of diabetes and its associated complications. ![]()
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